Cat.No.
zwf-01E
Species
E. coli
Product Name
Recombinant E. coli zwf Protein
Product Overview
Recombinant E. coli zwf Protein (1-491aa) without tag was expressed in E. coli.
Description
Glucose-6-phosphate dehydrogenase (G6PD) is the rate-limiting enzyme of the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of NADPH. G6PD converts glucose-6-phosphate into 6-phosphoglucono-delta-lactone and simultaneously produce NADPH. The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. G6PD deficiency cause acute hemolytic anemia.
Source
E. coli
Form
Liquid
Bio-activity
Specific activity is > 50 unit/mg obtained by measuring the increase of beta-NADPH in absorbance at 340 nm resulting from the reduction of beta-NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25 centigrade.
Molecular Mass
55.7 kDa
AA Sequence
MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTRIIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRLGAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEINDQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVGIEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDRSNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFYLRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPGLDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVDSITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE
Purity
> 95% by SDS-PAGE
Applications
Enzyme Activity, SDS-PAGE
Storage
Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
Concentration
1mg/ml (determined by Bradford assay)
Storage Buffer
Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 0.1mM PMSF, 2mM EDTA, 0.5mM DTT
Official Symbol
Synonyms
zwf; NADP(+)-dependent glucose-6-phosphate dehydrogenase; ECK1853; NADP(+)-dependent glucose-6-phosphate dehydrogenase; EC 1.1.1.49; Glucose-6-phosphate 1-dehydrogenase; Glucose-6-phosphate 1-dehydrogenase; Glucose-6-phosphate 1-dehydrogenase G6PD; G6PD1; G6pdx; Glucose 6 phosphate 1 dehydrogenase; MET19; POS10; Zwf1p
Gene ID
Protein Refseq
UniProt ID
Figure 1
zwf-01E, 1.jpg
Figure Note 1
3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Figure Title 1
SDS-PAGE
