Cat.No.
ung-61E
Species
E. coli
Product Name
Recombinant E. coli Uracil DNA Glycosylase
Product Overview
A recombinant?E. coli?strain carrying the Uracil DNA Glycosylase gene from?E. coli?K-12.
Description
Uracil-DNA Glycosylase catalyzes the hydrolysis of the N-glycosylic bond between the uracil and sugar, leaving an abasic site in uracil-containing single or double-stranded DNA. The enzyme shows no measurable activity on short oligonucleotides (<6 bases),="" or="" rna="">
Source
E. coli
Bio-activity
77,000 U/mg
Molecular Mass
25,693 Daltons
Purity
>99% by SDS-PAGE
Unit Definition
1 unit is defined as the amount of enzyme that catalyzes the release of 1.8 nmol of Uracil in 30 minutes from double-stranded, tritiated, Uracil containing-DNA at 37°C in 1X UDG Reaction Buffer.
Storage
-15 to -25°C
Concentration
2,000 U/ml
Storage Buffer
Supplied in: 10mM Tris-HCl, 50mM NaCl, 1mM DTT, 0.1mM EDTA, 50% glycerol pH 7.5 @ 25°C.Supplied with: 10X UDG Reaction Buffer: 200mM Tris-HCl, 10mM DTT, 10mM EDTA pH 8.0 @ 25°C.
Official Symbol
ung
Synonyms
ung; uracil-DNA-glycosylase; ECK2578; JW2564; NP_417075.1; EC 3.2.2.-
Gene ID
Protein Refseq
