Cat.No.
ruvC-133E
Species
E. coli
Product Name
Recombinant E. coli RuvC
Product Overview
Full-length recombinant protein expressed in E.coli and highly purified by combined chromatography
Description
E. coli RuvC protein (19 kDa) is a structurally specific endonuclease which binds specifically to the Holliday structure, an intermediate of recombination, at the late stage of homologous recombination and recombination repair and introduces a nick in the symmetrical point of the Holliday junction cleaving and resolving the recombinant. Functional form is dimmer.
Source
E. coli
Form
50% glycerol, 10 mM Tris-HCl (pH7.5), 2 mM EDTA, 100 mM NaCl, 5 mM mercaptoethanol
Purity
Over 90% by SDS-PAGE (CBB staining)
Applications
1) Functional studies in vitro. RuvC cleaves recombination intermediate at Holliday Junction. 2) SDS-PAGE3) Standard antigen for western blotting and ELISA
Storage
Sent at 4℃or at -20℃ and upon arrival, centrifuge briefly and store at -20℃ or -80℃ for longer period.
Concentration
1.0 mg/ml (determined by BCA method)
