Cat.No.
rnh-63E
Species
E. coli
Product Name
Recombinant E. coli RNase H
Product Overview
A recombinant?E. coli?strain carrying the RNAse H (rnh) gene from?E.coli.
Description
E.coli?RNase H (rnh) is an endoribonuclease which degrades the RNA strand of RNA/DNA hybrid molecules. RNase H digestion produces ribonucleotide molecules with 5-phosphate and 3-hydroxyl termini. RNAse H is nearly inactive against single or double-stranded RNA molecules.
Source
E. coli
Bio-activity
625,000 U/mg
Purity
>99% by SDS-PAGE
Unit Definition
1 unit is defined as the amount of enzyme that will hydrolyze 1 nmol of RNA from an 3H-labeled DNA:RNA hybrid molecule into acid-soluble material in 20 minutes at 37°C.
Storage
-25 to -15°C
Concentration
5,000 U/ml
Storage Buffer
Supplied in: 20 mM Tris-HCl, 100 mM KCl, 10 mM MgCl2, 0.1 mM EDTA, 0.1 mM DTT, 50% glycerol, pH 7.9 @ 25°C Supplied with: 10X RNAse H Buffer: 500mM Tris-HCl, 750 mM KCl, 30 mM MgCl2, 100 mM DTT, pH 8.3 @ 25°C.
