Cat.No.
PF1-62E
Species
E. coli
Product Name
Recombinant E. coli Poly(A) Polymerase
Product Overview
The gene encoding?E. coli?Poly(A) Polymerase expressed from a plasmid in?E. coli
Description
Poly(A) Polymerase catalyzes the addition of AMP from ATP to the 3′ hydroxyl of RNA. The reaction requires Mg2+ and is template independent.
Source
E. coli
Bio-activity
>20,000 U/mg
Molecular Mass
53,870 Daltons
Purity
>95% by SDS-PAGE
Unit Definition
1 unit is defined as the amount of enzyme that will incorporate 1 nmol of ATP into acid-insoluble material in 10 minutes at 37°C.
Storage
-25 to -15°C
Concentration
5,000 U/mL
Storage Buffer
Supplied in: 25mM Tris-HCl, 500mM NaCl, 0.1mM DTT, 0.1mM EDTA, 1mM MgCl2, 50% glycerol pH 8.0 @ 25°C. Supplied with: 10X Poly(A) Polymerase Reaction Buffer: 500mM Tris-HCl, 2.5M NaCl, 100mM MgCl2 pH 7.9 @ 25°C.
