Cat.No.
mug-01E
Species
E. coli
Product Name
Recombinant E. coli mug Protein, His-tagged
Product Overview
Recombinant E. coli mug protein(1-168), fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Description
Excises ethenocytosine and uracil, which can arise by alkylation or deamination of cytosine, respectively, from the corresponding mispairs with guanine in ds-DNA. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired base. The complementary strand guanine functions in substrate recognition. Required for DNA damage lesion repair in stationary-phase cells.
Source
E. coli
Tag
His
Molecular Mass
21.1 kDa
Protein length
1-168
AA Sequence
MGSSHHHHHHSSGLVPRGSHMGSMVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQAGFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELDQALVVRGR
Purity
>90% by SDS-PAGE
Stability
Shelf life: one year from despatch
Storage
Store undiluted at 2-8 centigrade for one week or (in aliquots) at -20 to -80 centigrade for longer. Avoid repeated freezing and thawing.
Storage Buffer
Presentation State: Purified
State: Liquid purified protein
Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol
State: Liquid purified protein
Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol
Gene Name
Official Symbol
Synonyms
mug; stationary phase mismatch/uracil DNA glycosylase; ECK3058; ygjF; stationary phase mismatch/uracil DNA glycosylase; EC 3.2.2.28
Gene ID
Protein Refseq
UniProt ID
Figure 1
mug-01E, 1.jpg
Figure Title 1
SDS-PAGE
