Cat.No.
mCherry-02E
Species
E. coli
Product Name
Recombinant E. coli mCherry Protein, His-tagged
Product Overview
Purified fluorescent protein mCherry, with N-terminal HIS tag, expressed in E. coli.
Description
mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.
Source
E. coli
Tag
His
Bio-activity
WB standard.
Molecular Mass
26.5 kDa
AA Sequence
MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Stability
Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Storage
Store at -80 centigrade.
Concentration
>50 µg/mL as determined by microplate BCA method
Storage Buffer
PBS, pH7.4, 10% glycerol.
Figure 1
mCherry-02E, 1.jpg
Figure Title 1
SDS-PAGE
