Cat.No.
map-793E
Species
E. coli
Product Name
Recombinant E. coli map Protein, His-tagged
Product Overview
Recombinant E. coli map (Accession # NP_414710) Ala2-Glu264, fused with a C-terminal 10-His tag, was produced in E. coli
Source
E. coli
Tag
His
Predicted N Terminal
Ala2
Form
Supplied as a 0.2 µm filtered solution in Tris, NaCl and Glycerol.
Bio-activity
Measure by its ability to remove methionine from a fluorogenic peptide substrate H-Met-Gly-Pro-AMC. The resulting GP-AMC is cleaved by Recombinant Human DPPIV/CD26. The specific activity is >0.5 pmol/min/µg, as measured under the described conditions.
Molecular Mass
Recombinant E. coli map, His-tagged has a calculated MW of 31 kDa. In SDS-PAGE migrates as 36 kDa, reducing conditions.
Protein length
Ala2-Glu264
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
