Cat.No.
MaBP-389E
Species
E. coli
Product Name
Recombinant E. coli MaBP
Product Overview
Recombinant MaBP was expressed in E.coli and purified by conventional chromatography techniques.
Description
MaBP (Maltose Binding Protein) is a protein related with the maltose/maltodextrin system of Escherichia coli, which is responsible for the uptake and efficient catabolism of maltodextrins. It is a complex regulatory and transport system involving many proteins and protein complexes. MaBP has been used to increase the yield of its fusion partner in many cases. In addition, MaBP is often able to promote the solubility of polypeptides to which it is fused.
Source
E. coli
Form
Liquid in 20mM Tris pH 8.0
Molecular Mass
42 kDa (387 aa)
Protein length
1-387aa
AA Sequence
MKIEEGKLVI WINGDKGYNG LAEVGKKFEK DTGIKVTVEH PDKLEEKFPQ VAATGDGPDI IFWAHDRFGG YAQSGLLAEI TPDKAFQDKL YPFTWDAVRY NGKLIAYPIA VEALSLIYNK DLLPNPPKTW EEIPALDKEL KAKGKSALMF NLQEPYFTWP LIAADGGYAF KYENGKYDIK DVGVDNAGAK AGLTFLVDLI KNKHMNADTD YSIAEAAFNK GETAMTINGP WAWSNIDTSK VNYGVTVLPT FKGQPSKPFV GVLSAGINAA SPNKELAKEF LENYLLTDEG LEAVNKDKPL GAVALKSYEE ELAKDPRIAA TMENAQKGEI MPNIPQMSAF WYAVRTAVIN AASGRQTVDE ALKDAQTNSS SNNNNNNNNN NLGIEGR
Purity
> 95% by SDS - PAGE
Applications
SDS-PAGE
Storage
Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Concentration
1 mg/ml (determined by Bradford assay)