Cat.No.
LT-910E
Species
E. coli
Product Name
Recombinant E. coli LT-IIa, His-tagged
Product Overview
Type II heat-labile enterotoxin, LT-IIa, from Escherichia coli was produced recombinantly in E. coli and purified to homogeneity using a combination of nickel affinity and gel filtration chromatography. Protein has been His-tagged at the COOH ends of each of the five B subunits.
Description
LT-IIa is a type II heat-labile enterotoxin produced by certain strains of Escherichia coli. The enterotoxin binds to GD1b, GD1a, GM1, and to several other gangliosides on the cell surface. The molecule induces high levels of cAMP in the cell upon intoxication. The molecule is also a very strong mucosal and systemic adjuvant when administered by peritoneal, intranasal, or intradermal routes. Single point substitution mutants of LT-IIa are available from Dr. T.D. Connell, upon request, that have altered ganglioside-binding and toxic activities.
Source
E. coli
Tag
His
Form
Powder
Molecular Mass
82kDa, (A subunit + five B subunits)
Purity
> 95%
Storage
Store at -20 centigrade (long term). Store at +4 centigrade when dissolved in aqueous solution (pH 7.0 to pH 7.4).
Concentration
5mg/ml
Storage Buffer
Water (pH 7.0 to 7.4)
Gene Name
Heat-Labile Enterotoxin LT-IIa
Official Symbol
LT
Synonyms
Type II heat-labile enterotoxin; Heat-Labile Enterotoxin; LT toxin; LT-Iia
