Cat.No.
RuvC-2666E
Species
E. coli
Product Name
Recombinant E. coli Holliday Junction DNA Helicase RuvC
Product Overview
RecombinantE. coliHolliday Junction DNA Helicase RuvC is expressed in E. coli MW=19KDa.
Description
E. coli RuvC protein is a structurally specific endonuclease which binds specifically to the Holliday structure, an intermediate of recombination, at the late stage of homologous recombination and recombination repair and introduces a nick in the symmetrical point of the Holliday junction cleaving and resolving the recombinant.
Source
E. coli
Form
50% glycerol, 10 mM Tris-HCl (pH7.5), 2 mM EDTA, 100 mM NaCl, 5 mM mercaptoethanol.
Purity
RuvC protein over 90% by SDS-PAGE (CBB staining).
Usage
1) Studies on homologous recombination mechanism. 2) To use as endonuclease which functions specifically to the Holliday structure.
Storage
-200C.
Concentration
1.0 mg/ml (determined by BCA method).
Gene Name
Synonyms
ruvC; Crossover junction endodeoxyribonuclease ruvC
Gene ID
Protein Refseq
