Cat.No.
RuvB-2665E
Species
E. coli
Product Name
Recombinant E. coli Holliday Junction DNA Helicase RuvB
Product Overview
Recombinant E. coli Holliday Junction DNA Helicase RuvB is expressed inE. coli
Description
E. coli RuvB protein forms a complex with RuvA protein at the late stage of homologous recombination and recombination repair and binds specifically to the Holliday structure which is the intermediate of recombination, allowing the migration of Holliday junction using ATP hydrolysis energy and expands the heteroduplex region. RuvB forms a hexamer ring structure and surrounds the double chain DNA and covers RuvA tetramer bound to the Holliday junction from both sides. RuvB is a DNA motor protein which possesses the ATPase activity, activated by DNA and RuvA protein. Its molecular weight is 37 kD and forms a dimer in solution in the physiological condition.
Source
E. coli
Form
50% glycerol, 10 mM Tris-HCl (pH7.5), 2 mM EDTA, 100 mM NaCl, 5 mM mercaptoethanol.
Purity
RuvB protein over 90% by SDS-PAGE (CBB staining).
Usage
1) Studies on homologous recombination mechanism. 2) To make use of the motor protein function that specifically migrates the Holliday junction by forming a complex with RuvA (branch-migration protein).
Storage
-200C.
Concentration
1.0 mg/ml (determined by BCA method).
Synonyms
ruvB; Holliday junction DNA helicase RuvB
Gene ID
Protein Refseq
UniProt ID
