Cat.No.
FABI-784E
Species
E. coli
Product Name
Recombinant E. coli FABI Protein, His-tagged
Product Overview
Recombinant E.coli FABI (NP_287908.1) (Gly 2-Lys 262) was expressed, with a polyhistide tag at the N-terminus.
Source
E. coli
Tag
His
Predicted N Terminal
Met
Form
Lyophilized from sterile 20mM Tris 0.5M NaCl, 10% glycerol, pH 7.5, 5% ~ 8% trehalose and mannitol.
Molecular Mass
The recombinant E.coli FABI consisting of 276 amino acids and has a calculated molecular mass of 29.5 kDa. It migrates as an approximately 33 kDa band in SDS-PAGE under reducing conditions.
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
>98 % as determined by SDS-PAGE
Stability
Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
