Cat.No.
eco-794E
Species
E. coli
Product Name
Recombinant E. coli eco Protein, His-tagged
Product Overview
Recombinant E. coli eco (Accession # NP_416713) Ala21-Arg162, fused with a C-terminal 6-His tag, was produced in E. coli
Source
E. coli
Tag
His
Predicted N Terminal
Ala21
Form
Lyophilized from a 0.2 µm filtered solution in Tris and NaCl.
Bio-activity
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2.The IC50 value is <1.0 nM, as measured under the described conditions.
Molecular Mass
Recombinant E. coli eco, His-tagged has a calculated MW of 17 kDa. In SDS-PAGE migrates as 18 kDa, reducing conditions.
Protein length
Ala21-Arg162
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution
Reconstitute at 250 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
