Cat.No.
DnaK-313E
Species
E. coli
Product Name
Recombinant E. coli Dnak Protein
Product Overview
DnaK (amino acids 1-638) was amplified by PCR and cloned into an E. coli expression vector. Recombinant E. coli DnaK (1-638) was overexpressed in E. coli and was purified to apparent homogeneity by using conventional column chromatography techniques.
Description
ATP-regulated binding and release of polypeptide substrates. Hsc56 exhibits specificity toward Hsc62, as Hsc56 does not activate DnaK or Hsc66 ATPase activity.
Source
E. coli
Form
Liquid
Molecular Mass
69 kDa (638aa)
AA Sequence
MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQDGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAWVEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRIAGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEVLATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSAQQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDIDDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLLDVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAADNKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLNEDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIESALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDDDVVDAEFEEVKDKK
Purity
> 85% by SDS-PAGE
Applications
SDS-PAGE
Storage
Can be stored at 2 to 8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
Concentration
1 mg/mL (determined by Bradford assay)
Storage Buffer
25mM Tris-HCl buffer (pH 7.5) containing 100mM NaCl, 5mM DTT,10%glycerol
Official Symbol
Synonyms
dnaK; chaperone protein DnaK; ECK0014; groPAB; groPC; groPF; grpC; grpF; seg; chaperone protein DnaK;
Gene ID
Protein Refseq
UniProt ID
Figure 1
dnaK-313E, 1.jpg
Figure Note 1
3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Figure Title 1
SDS-PAGE
