Cat.No.
degP-792E
Species
E. coli
Product Name
Recombinant E. coli degP Protein, His-tagged
Product Overview
Recombinant E. coli degP (Accession # NP_414703) Ala27-Gln474, fused with a C-terminal 10 His tag and ERSIGS sequence, was produced in E. coli
Source
E. coli
Tag
His
Predicted N Terminal
Ala27
Form
Supplied as a 0.2 µm filtered solution in HEPES and NaCl.
Bio-activity
Measured by its ability to cleave beta -casein.Recombinant E. coli HTRA/Protease Do will cleave >95% cleavage of beta -casein, as measured under the described conditions.
Molecular Mass
Recombinant E. coli degP, His-tagged has a calculated MW of 49 kDa. In SDS-PAGE migrates as 50 kDa, 41 kDa and 27 kDa, reducing conditions.
Protein length
Ala27-Gln474
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
