Cat.No.
CFP-002E
Species
E. coli
Product Name
Recombinant Cyan Fluorescent Protein, His-tagged
Product Overview
The recombinant CFP (Cyan Fluorescent Protein) is expressed and purified from transformed E. coli using a method that ensures high purity and maximal CFP fluorescence. Endotoxin has been removed, so the protein is suitable for in vivo injection or cell culture applications. The protein is a 31.3 kDa monomer with 284 amino acids and an N-terminal His-tag, Ex./Em. = 458/480 nm, extinction coefficient 26000M^-1CM^-1.
Fluorescent protein ideal for subcellular labeling/visualization.
Fluorescent protein ideal for subcellular labeling/visualization.
Description
Cyan Fluorescent Protein (CFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo.
Source
E. coli
Tag
His
Form
Lyophilized (Freeze Dried)
Molecular Mass
31.3 kDa
AA Sequence
MSGGEELFAGIVPVLIELDGDVHGHKFSVRGEGEGDADYGKLEIKFICTTGKLPVPWPTLVTTLAWGIQCFARYPEHMKMNDFFKSAMPEGYIQERTIHFQDDGKYKTRGEVKFEGDTLVNRVELKGEGFKEDGNILGHKLEYSAISDNVYIMPDKANNGLEANFKIRHNIEGGGVQLADHYQTNVPLGDGPVLIPINHYLSCQSAISKDRNEARDHMVLLESFSAYCHTHGMDELYRSGLRSRAQASNSAVDGTAGPGSTGSR
Endotoxin
<0.1 ng/μg
Purity
≥97%
Applications
The protein is suitable as a control reagent for CFP expression studies or as a labeling reagent. Applications include: Use as standards for SDS-PAGE, Western blot analysis of CFP transfected cells, label other proteins, calibration of fluorometers and flow cytometers, fluorescence microscope, or microinjection of CFP into cells and tissues, etc. The recombinant CFP is ideal for fusion tag applications and is perfect for triple labeling with EGFP, RFP or YFP.
Usage
For Research Use Only! Not to be used in humans
Storage
At -20 centigrade.
At -80 centigrade for long-term storage.
At -80 centigrade for long-term storage.
Reconstitution
Reconstitute with dH₂O to 1 mg/mL
Handling
Centrifuge the vial prior to opening.
Synonyms
CFP, Cyan Fluorescent Protein
