Cat.No.
toxB-12C
Species
C. difficile
Product Name
Recombinant C. difficile toxB, His-tagged
Product Overview
Recombinant C. difficile toxB (Met1-Leu543), fused with a C-terminal 6-His tag, was expressed in E. coli
Description
Clostridium difficile toxin B is a toxin produced by the bacteria Clostridium difficile. C. difficile produces two major kinds of toxins that are very potent and lethal; an enterotoxin (Toxin A) and a cytotoxin (Toxin B, this protein).
Source
E. coli
Tag
His
Predicted N Terminal
Ser2
Form
Supplied as a 0.2 µm filtered solution in Tris and NaCl.
Bio-activity
Measured by its ability to hydrolyze UDP-Glucose.
Molecular Mass
Predicted Molecular Mass: 64 kDaSDS-PAGE: 60-65 kDa, reducing conditions
Endotoxin
< 1.0 EU per 1 μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
Storage
Avoid repeated freeze-thaw cycles.6 months from date of receipt, -20 to -70 °C as supplied.3 months, -20 to -70 °C under sterile conditions after opening.
