Cat.No.
Fla-012B
Species
Borrelia burgdorferi
Product Name
Recombinant Borrelia garinii p14 flagellin Protein, His-tagged
Product Overview
Recombinant Borrelia garinii p14 antigen produced from the internal fragment (amino acids 197-274) of the 41 kDa flagellin protein. Contains a His-tag at the N-terminus. Molecular weight calculated from the amino acid sequence is 16.4 kDa.
Source
E. coli
Tag
His
Form
Purified, Liquid
Molecular Mass
16.4 kDa
Protein length
amino acids 197-274
Purity
> 95% pure (SDS-PAGE)
Applications
Suitable for use in ELISA (3-11 µg/mL), Lateral Flow assays, Dot Blot and Western Blot. It has also been used as a positive control in ELISA, CLIA and Lateral Flow assays. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Storage
Store at -20 to -80centigrade. Aliquot to avoid multiple freeze/thaw cycles.
Concentration
1.307 mg/mL (Bradford Method)
Storage Buffer
20 mM Phosphate, pH 8.0, 1 M NaCl, 0.1% Polyoxyethylene (10) Tridecylether.
