Cat.No.
VlsE-006B
Species
Borrelia burgdorferi
Product Name
Recombinant Borrelia burgdorferi VlsE Protein, His-tagged
Product Overview
Recombinant Borrelia burgdorferi VlsE antigen produced from the complete ORF of the variable major protein-like sequence expressed. Contains a His-tag at the N-terminus. Relative molecular weight calculated from the amino acid sequence is 36,678.82 Da.
Source
E. coli
Tag
His
Form
Purified, Liquid
Molecular Mass
36.7 kDa
Purity
> 95% pure (SDS-PAGE)
Applications
Suitable for IgG and IgM detection in ELISA (0.32 µg/mL), Dot Blot and Western Blot. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Storage
Store at -20centigrade. Aliquot to avoid multiple freeze/thaw cycles.
Concentration
1.014 mg/mL (Bradford Method)
Storage Buffer
20 mM Phosphate buffer pH 7, 0.1 M KCI, 0.1% Polyoxyethylene (10) Tridecylether.
