Cat.No.
cya-140B
Species
Bordetella pertussis
Product Name
Recombinant Bordetella pertussis Adenylate Cyclase
Product Overview
This product is a recombinant toxin of Bordetella pertussis Tohama strain expressed in E. coli from the structural gene cyaA and its activator gene cyaC.
Description
Adenylate cyclase is a toxin produced by a gram-negative coccobacillus, Bordetella pertusis. This toxin penetrates into the animal cells and produces cAMP from ATP in the cytoplasm. It belongs to the RTX family of toxins produced by many gram-negative bacteria. The molecular weight is 177 kDa.
Source
E. coli
Form
550 μｇ/ml in 50 mM Tris-HCl(pH8.0), 6M urea, 150 mM NaCl
Bio-activity
Addition of the 10~100 ng/ml adenylate cyclase toxin to medium increased significant levels of cellular cAMP levels in CHO and human monocyte cells.
Purity
More than 90% purity by SDS-PAGE (CBB staining)
Applications
1) Functionale studies on the cAMP- signaling pathway (10~100 ng/ml) 2) Antigen for western blotting and ELISA 3) SDS-PAGE marker as native adenylate cyclase
Storage
Shipped with dry-ice and store at -80℃
