Cat.No.
BFP-001E
Species
E. coli
Product Name
Recombinant Blue Fluorescent Protein, His-tagged
Product Overview
The recombinant Blue Fluorescent Protein (BFP) is expressed and purified from transformed E. coli using a method that ensures high purity and maximal BFP fluorescence. The protein is a 29 kDa monomer with 259 amino acids, pI: 6.17. Ex.= 308-383 nm; Em.= 440-447 nm. The protein is engineered with 6xHis-tag on the N-terminal, which can be used for detection with anti-His-Tag antibody, or protein removal by using Ni++ beads.
Description
Blue fluorescent protein (BFP) is a mutant of green fluorescent protein (GFP), where the chromophore has been modified to shift the emitted fluorescence into the blue spectral region.
Source
E. coli
Tag
His
Form
Lyophilized (Freeze Dried)
Molecular Mass
29.0 kDa
AA Sequence
MGSSHHHHHHSSGLVPRGSHMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLSHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK
Endotoxin
<0.1 ng/μg
Purity
≥97%
Applications
The protein is suitable as a control reagent for BFP expression studies or as a labeling reagent. Applications include: Use as standards for SDS-PAGE, Western blot analysis of BFP transfected cells, label other proteins, calibration of fluorometers and flow cytometers, fluorescence microscope, or microinjection of BFP into cells and tissues, etc. The recombinant BFP is ideal for fusion tag applications and is perfect for triple labeling with EGFP, RFP, YFP, or any other dyes. The 6 × His-Tag on the N-terminal can be used in Western blot detection with 6 × His-tag antibodies. The 6 × His-tag can also be used in removal or purification of the BFP.
Usage
For Research Use Only! Not to be used in humans
Storage
At -20 centigrade.
At -80 centigrade for long-term storage.
At -80 centigrade for long-term storage.
Reconstitution
Reconstitute with dH2O to 1 mg/mL
Handling
Centrifuge the vial prior to opening.
Synonyms
BFP, Blue Fluorescent Protein
