Cat.No.
OmpA-249B
Species
Others
Product Name
Recombinant Bacterial Outer Membrane Protein-A
Product Overview
The recombinant form was found to be undistinguishable from the wild type when examined by SDS-PAGE and gel filtration chromatography yielding a 48 kDa monomeric protein. The immunological similarity of the protein samples was demonstrated by employing polyclonal and monoclonal antibodies in ELISA and Western Blot techniques. All forms of A-protein were found to activate the secretion of tumour necrosis factor alpha from murine macrophage. The OmpA is purified by proprietary chromatographic techniques. The species specific structural gene for the monomeric form of A-protein was cloned into a pET-3d plasmid in order to express and produce a recombinant form of the protein inE. coliBL21 (DE3).
Description
Achromogenic atypical Aeromonas salmonicida is the causative agent of goldfish ulcer disease. Virulence of this bacterium is associated with the production of a paracrystalline outer membrane A-layer protein. The induced protein was isolated from inclusion bodies by a simple solubilization-renaturation procedure and purified by ion exchange chromatography on Q-Sepharose to over 95% pure monomeric protein. Recombinant A-protein was compared by biochemical, immunological and molecular methods with the A-protein isolated from atypical A. salmonicida bacterial cells by the glycine and the membrane extraction methods.
Source
E. coli
Form
It is recommended to reconstitute the lyophilized OmpA in sterile 0.4% NaHCO3.
Purity
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Stability
Lyophilized Bacterial Outer Membrane Protein-A although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon C between 2-7 days and for future use reconstitution OmpA should be stored at 4 below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Synonyms
ompA; Bacterial outer membrane protein; outer membrane protein A
Gene ID
Protein Refseq
Figure 1
productimages/ompa-249b, ompa.gif
Figure Title 1
PDB rendering based on 1g90.
