Cat.No.
Cry1ac-338B
Species
Bacillus thuringiensis
Product Name
Recombinant Bacillus thuringiensis Cry1ac Protein, His-tagged
Product Overview
Recombinant Bacillus Cry1ac (AAU87037.1) (Met1-Glu1178) was expressed with a polyhistidine tag at the N-terminus.
Source
E. coli
Tag
His
Predicted N Terminal
Met
Form
Lyophilized from sterile 50 mM Na2CO3, 10 mM DTT, pH 9.5. Normally 5 %- 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Molecular Mass
The recombinant bacillus Cry1ac consists of 1189 amino acids and predicts a molecular mass of 134.9 kDa.
Protein length
Met1-Glu1178
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Purity
> (81.2+6.2) % as determined by SDS-PAGE.
Storage
Samples are stable for up to twelve months from date of receipt at-70 centigrade. Store it under sterile conditions at-20 centigrade to-80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Official Symbol
Cry1ac
Synonyms
Cry1ac;Cry1ac Protein; Bacillus thuringiensis
