Cat.No.
OGA-85B
Species
B. thetaiotaomicron
Product Name
Recombinant B. thetaiotaomicron OGA, His-tagged
Product Overview
Recombinant B. thetaiotaomicron OGA(Gln22-Lys737), fused with an N-terminal Met and 6-His tag, was expressed in E. coli
Description
Protein O-GlcNAcase (EC 3.2.1.169, OGA, glycoside hydrolase O-GlcNAcase, O-GlcNAcase, BtGH84, O-GlcNAc hydrolase) is an enzyme with system name (protein)-3-O-(N-acetyl-D-glucosaminyl)-L-serine/threonine N-acetylglucosaminyl hydrolase.
Source
E. coli
Tag
His
Predicted N Terminal
Met
Form
Supplied as a 0.2 µm filtered solution in Tris, NaCl, Brij and Glycerol.
Bio-activity
Measured by its ability to hydrolyze 4-methylumbelliferyl-N-acetyl-beta -D-glucosaminide (4-MU-GlcNAc) The specific activity is >3500 pmol/min/µg.
Molecular Mass
Predicted Molecular Mass:83 kDaSDS-PAGE:66-76 kDa, reducing conditions
Endotoxin
< 1.0 EU per 1 μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
Storage
Avoid repeated freeze-thaw cycles.6 months from date of receipt, -20 to -70 ºC as supplied.3 months, -20 to -70 ºC under sterile conditions after opening.
