Cat.No.
cyaA-001B
Species
B. pertussis
Product Name
Recombinant B. pertussi cyaA Protein
Product Overview
This product is a recombinant toxin of Bordetella pertussis Tohama strain expressed in E. coli from the structural gene cyaA and its activator gene cyaC and purified by the method of Friedman.
Description
Adenylate cyclase is a toxin produced by a gram-negative coccobacillus, Bordetella pertusis. This toxin penetrates into the animal cells and produces cAMP from ATP in the cytoplasm. It belongs to the RTX family of toxins produced by many gram-negative bacteria.
Source
E. coli
Form
550 μｇ/ml in 50 mM Tris-HCl(pH8.0), 6M urea, 150 mM NaCl
Bio-activity
Addition of the 10~100 ng/ml adenylate cyclase toxin to medium increased significant levels of cellular cAMP levels in CHO and human monocyte cells as examined by Biotrak cAMP EIA System of GE Healthcare Bioscience.
Molecular Mass
177 KDa
Purity
More than 90% purity by SDS-PAGE (CBB staining)
Applications
1) Functional studies on the cAMP- signaling pathway (10~100 ng/ml)
2) Antigen for western blotting and ELISA
3) SDS-PAGE marker as native adenylate cyclase
2) Antigen for western blotting and ELISA
3) SDS-PAGE marker as native adenylate cyclase
Storage
Shipped with dry-ice and store at -80centigrade.
Warning
For research use only, not to be used for human application.
Official Symbol
Synonyms
cyaA; bifunctional hemolysin-adenylate cyclase; cya
Gene ID
Protein Refseq
UniProt ID
Figure 1
cya-001B, 1.jpg
Figure Title 1
SDS polyacrylamide gel electrophoresis of adenylate cyclase toxin.
