Cat.No.
CED40
Species
Aeromonas spp.
Product Name
Recombinant Aeromonas CED40 protein
Product Overview
Recombinant Aeromonas CED40 protein was expressed in Escherichia coli.
Source
E. coli
Form
Lyophilized from a 0.2μm filtered concentrated solution in 20 mM Tris, 150 mM NaCl, 5μM ZnSO4, pH 8.0.
Bio-activity
Sequentially cleaves N-terminal amino acids except E, D, and X-P.
Molecular Mass
Approximately 31.4 kDa, a single non-glycosylated polypeptide chain containing 291 amino acids.
Protein length
291
AA Sequence
MPPITQQATVTAWLPQVDASQITGTISSLESFTNRFYTTTSGAQASDWIASEWQALSASLPNASVKQVSHSGYNQKSVVMTITGSEAPDEWIVIGGHLDSTIGSHTNEQSVAPGADDDASGIAAVTEVIRVLSENNFQPKRSIAFMAYAAEEVGLRGSQDLANQYKSEGKNVVSALQLDMTNYKGSAQDVVFITDYTDSNFTQYLTQLMDEYLPSLTYGFDTCGYACSDHASWHNAGYPAAMPFESKFNDYNPRIHTTQDTLANSDPTGSHAKKFTQLGLAYAIEMGSATG
Endotoxin
Less than 0.1 EU/µg of rAeromonas Aminopeptidase as determined by LAL method.
Purity
>98% by SDS-PAGE and HPLC analysis.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.
UniProt ID
Figure 1
CED40, 1.jpg
Figure Title 1
SDS-PAGE of CED40
