Cat.No.
APALIM-1811A
Species
Acetobacter Pasteurianus
Product Name
Recombinant Acetobacter Pasteurianus APALIM Protein (1-429 aa), His-tagged
Product Overview
Recombinant Acetobacter Pasteurianus (Acetobacter turbidans) APALIM Protein (1-429 aa) is produced by Yeast expression system. This protein is fused with a 6xHis tag at the N-terminal. Research Area: Microbiology. Protein Description: Full Length.
Description
This methylase recognizes the double-stranded sequence GTGCAC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the ApaLI endonuclease.
Source
Yeast
Tag
His
Form
Tris-based buffer,50% glycerol
Molecular Mass
48.5 kDa
Protein length
1-429 aa
AA Sequence
MNKDEVVVSLFAGAGGFSSGFSQAGLKPLFGAEINADACQTYQENVGSPCHQLDLSTVDPSHIEMLTGGKRPFVVIGGPPCQGFSTAGPRNFADPRNLLIFNYLNIVERLSPRWLIFENVEGLLTSGGGRDLARLVREFVDMGYSVRLQKVNLAAYGVPQTRKRVLIIGNRLGIDFQFPEELYSFDSGKAKKASGKPLAPSLAEAVAGLGPAASDKDALVPYASSEPVNAFDARMRAGNRVEVVTHHVRVEAAERMQVELLKPGQTMKDLPPELWHESYRRRANRRVSDGTPTEKRGGAPSGIKRLHGNLQSLTITGPAAREFIHPTEHRPLTIRECARIQTFPDKYRWVGNNASVIQQIGNAVPPLAAERLAKHLRDIDGSFGADTRPAGAMSAKLLGFVLTEALGMSPALKSTEALLAEMHQGGFVF
Purity
> 90% as determined by SDS-PAGE.
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
Concentration
A hardcopy of COA with reconstitution instruction is sent along with the products.
Synonyms
apaLIM; Cytosine-specific methyltransferase ApaLI;
UniProt ID
Figure 1
APALIM-1811A, 1.jpg
Figure Note 1
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
