Cat.No.
Lysostaphin-91S
Species
Staphylococcus staphylolyticus
Product Name
Native Staphylococcus staphylolyticus Lysostaphin
Product Overview
Native Lysostaphin was purified from Staphylococcus staphylolyticus. Protein 50-70 % by biuret.
Description
Lysostaphin is a lytic enzyme isolated from a bacterial culture of Staphylococcus staphylolyticus, which lyses Staphylococcus species, including S. aureus. Lysostaphin has hexosaminidase activity (specific for the glucosaminyl-muramic acid bond of the bacterial carbohydrate backbone, but does not react with S. aureus), amidase activity, and endopeptidase activity, which cleaves polyglycine cross-links in the cellular wall of Staphylococcus species.
Source
Staphylococcus staphylolyticus
Form
lyophilized powder
Bio-activity
≥500 units/mg protein
Molecular Mass
~25 kDa
Unit Definition
One unit will reduce the turbidity (A620) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 at 37°C in a 6.0 ml reaction mixture. pH Optimum for activity: ~7.5.
Storage
Store the product at –20 °C. When stored at –20 °C, the enzyme retains activity for at least 3 years.
