Cat.No.
Endoproteinase Lys-C-85L
Species
Lysobacter enzymogenes
Product Name
Native Lysobacter enzymogenes Endoproteinase Lys-C
Product Overview
Native Endoproteinase Lys-C was purified from Lysobacter enzymogenes.
Description
Endoproteinase Lys-C is a protease that cleaves proteins on the C-terminal side of lysine amino acids. Lys-C activity is optimal in pH range of 7.0 - 9.0.
Source
Lysobacter enzymogenes
Form
lyophilized powder
Unit Definition
One unit will hydrolyze 1.0 μmole of N-p-tosyl-Gly-Pro-Lys p-nitroanilide per min at pH 7.7 at 25 °C.
Applications
Endoproteinase Lys-C from Lysobacter enzymogenes is useful in the determination of primary structures of proteins. It has been used in a study to investigate the evidence for trisulfide bonds in a recombinant variant of a human IgG2 monoclonal antibody. Endoproteinase Lys-C has been used for the digestion monoclonal antibodies for disulfide bond assignment.
Storage
2-8°C
