Cat.No.
CRM197-190
Species
Corynebacterium diphtheriae
Product Name
Native Diphtheria Toxin CRM197, Mutant [Glu52]
Product Overview
CRM197 was expressed in Corynebacterium diphtheriae, and purified by state-of-art protein purification method such as centrifugation, amonium sulfate precipitation and chromatography, then lyophilized after addition of sucrose. Moisture: <3% by Karl-Fisher
Description
The Glu52 diptheria toxin, also known as CRM197 (Cross-reactive materials 197), is a nontoxic mutant of diphtheria toxin, has a mutation in fragment A (a glycine to glutamic acid substitution at position 52) that results in the complete loss of enzymatic activity.
Source
Corynebacterium diphtheriae
Form
Lyophilized powder (liquid also could be provided)
Molecular Mass
~58 kDa
Sterility
Inoculation/membrane filtration
Endotoxin
<10 EU/μg of protein by LAL method.
Purity
> 90% by SDS-PAGE and HPLC
Storage
Store at -20 centigrade.
Concentration
1~20mg/ml(aq) by BCA or Lowry
Reconstitution
Before lyophilization, CRM197(aq) was about 5mg/ml in sterile water with 5% sucrose. Vials containing appoximately 5mL of CRM197(aq) were lyophilized. The lyophilized powder can be reconstituted with water or sodium phosphate buffer. We could provide liquid or lyophilized powder.
Figure 1
CRM197-190, 1.jpg
Figure Note 1
Fig 1 A. Purity analysis of CRM197 by SDS PAGE (10%); B. Identity test of CRM197 by mass spectrometry, Mascot score histogram(top), sequence coverage (bottom); C. Identity test of CRM197 by western blotting; D. Purity analysis of CRM197 by HPLC.
