Cat.No.
dnt-142B
Species
B. bronchiseptica
Product Name
Native Bordetella bronchiseptica Dermonecrotic Toxin
Product Overview
DNT was purified from B. bronchiseptica strain S798 by propriety chromatography and differential precipitation method.
Description
Dermonecrotic toxin (DNT) is a common toxin produced by pathogenic bacteria belonging to the genus Bordetella, such as B. pertussis, B. parapertussis, and B. bronchiseptica. This toxin activates small GTP-binding proteins such as Rho, Rac and Cdc42 through deamidation or polyamination for downstream signaling pathways.
Source
B. bronchiseptica
Form
0.53 mg/ml of DNT in 50 mM sodium phosphate buffer (pH 7.2), 1 M urea, 0.3 M sodium sulfate, filter-sterilized.
Bio-activity
DNT at 5 ng/ml induces ballooning morphological change in cultured mouse osteoblastic cell line MC3T3-E1.
Purity
More than 95 % purity by SDS-PAGE (CBB staining)
Applications
1) For the studies of the signaling pathways mediated by small GTP-binding proteins. 2) Antigen to be used for detection of infection of Bordetella by immunological method.
Storage
Store at 4℃
