Cat.No.
AK-14B
Species
Bacillus stearothermophilus
Product Name
Native Bacillus stearothermophilus Acetate Kinase
Product Overview
Native Acetate Kinase was purified from Bacillus stearothermophilus. EC 2.7.2.1
Description
In molecular biology, acetate kinase (EC 2.7.2.1), which is predominantly found in micro-organisms, facilitates the production of acetyl-CoA by phosphorylating acetate in the presence of ATP and a divalent cation. The enzyme is important in the process of glycolysis, enzyme levels being increased in the presence of excess glucose. The growth of a bacterial mutant lacking acetate kinase has been shown to be inhibited by glucose, suggesting that the enzyme is involved in excretion of excess carbohydrate. A related enzyme, butyrate kinase, facilitates the formation of butyryl-CoA by phosphorylating butyrate in the presence of ATP to form butyryl phosphate.
Source
Bacillus stearothermophilus
Form
Lyophilized
Bio-activity
more than 1,100 U/mg protein
Molecular Mass
Molecular weight : ca. 160,000 Subunit molecular weight : ca. 40,000
Unit Definition
One unit of activity is defined as the amount of AK that forms 1 μmol of ADP per minute at 30°C.
Applications
The enzyme is useful for determination of acetate or for ATP regeneration system.
Stability
No detectable decrease in activity up to 65°C.
Storage
stable at -20°C for at least one year.
