Cat.No.
Lectin-1761A
Species
Agaricus bisporus
Product Name
Native Agaricus bisporus lectin Protein, Fluorescein labeled
Product Overview
This product is the Fluorescein labeled Agaricus bisporus lectin and has sugar specificity against Gal(b-1,4)GalNAc (T disaccharide). The excitation maximum is at 495 nm and the emission maximum is at 515 nm.
Description
Agaricus bisporus lectin (ABL) isolated from the edible white button mushroom, is composed of two to four very similar isolectins, each a tetramer of approximately 17 kD subunits. This lectin binds the Thomsen-Friedenreich antigen, also called T disaccharide, galactosyl (β-1,3) N-acetylgalactosamine. Unlike peanut agglutinin, which does not bind sialylated T antigen, ABL binds either sialylated or asialylated forms. Also, whereas peanut agglutinin has a proliferative effect on cancer cells, ABL is anti-proliferative, apparently without cytotoxicity. ABL binds all blood groups and O-linked glycans of IgA subclasses. Simple mono- and disaccharides are only marginally inhibitory.
Source
Agaricus bisporus
Form
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.08% sodium azide
Storage
Refrigerate in the dark.
Synonyms
Lectin; ABL
UniProt ID
Concentration
1 mg/ml
Bio-activity
Sugar Specificity: Gal(b-1,3)GalNAc (T disaccharide)
Applications
Immunofluorescence, Glycobiology
Application
Immunofluorescence, Glycobiology
