Cat.No.
RPL-262P
Species
Peptostreptococcus sp.
Product Name
Recombinant Peptostreptococcus RPL, His-tagged, Biotinylated
Product Overview
Biotinylated Protein L (Biotinylated Protein L) was labeled with biotin based on Protein L Protein, which was produced in E.coli cells.?
Description
Protein L was isolated from the surface of bacterial species Peptostreptococcus magnus and was found to bind Ig(IgG,IgM,IgA,IgE and IgD) through L chain interaction, from which the name was suggested. Despite this wide-ranging binding capability with respect to Ig classes, Protein?L is not a universal immunoglobilin-binding protein. Binding of Protein?L to immunoglobulins is restricted to those containing kappa light chains (i.e., k?chain of the VL domain). In humans and mice, kappa (k) light chains predominate. The remaining immunoglobulins have lambda (l) light chains. The recombinant protein contains four immunoglobulin (Ig) binding domains (Bdomains) of the native protein. Besides antibody, protein L is also suitable for binding of a wide range of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies (Dabs).
Source
E. coli
Tag
His
Form
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose are added as protectants before lyophilization.
Molecular Mass
Biotinylated Protein L, fused with the polyhistidine tag at N-terminus and a single cysteine at C-terminus. DTT-reduced protein migrates as a 36-38 kDa polypeptide .
Endotoxin
Less than 1.0 EU per μg of the Biotinylated Protein L by the LAL method.
Purity
>95% as determined by reduced SDS-PAGE.
Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).
