Cat.No.
tdh-01C
Species
Cupriavidus necator
Product Name
Recombinant Cupriavidus necator tdh Protein, His-tagged
Product Overview
Recombinant Cupriavidus necator tdh Protein with His tag was expressed in E.coli.
Description
Threonine dehydrogenase (EC 1.1.1.103) is a β-NAD+ coenzyme dependent enzyme, from the group of short chain alcohol dehydrogenases. It is a key enzyme in L threonine catabolism in microorganisms and mammals. The enzyme catalyzes conversion of l-threonine to 2-amino-3 ketobutyrate, with simultaneous reduction of NAD+.
Source
E. coli
Tag
His
Form
Lyophilized
Bio-activity
Specific activity is ≥ 2 U/mg
Molecular Mass
36.8 kDa
Purity
≥95%
Usage
For Research Use Only! Not For Use in Humans.
Storage
Store the lyophilized protein at -20 centigrade. Reconstituted enzyme can be stored in working aliquots at -20 centigrade. Avoid repeated freeze-thaw cycles.
Storage Buffer
Proprietary buffer
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in distilled water.
Synonyms
ThrDH; tdh; L-threonine 3-dehydrogenase; Threonine Dehydrogenase
UniProt ID
Figure 1
tdh-01C, 1.jpg
Figure Note 1
Recombinant Protein loaded under reducing conditions and stained with Coomasie Blue.
Figure Title 1
SDS-PAGE (4-20%) of recombinant ThrDH
Figure 2
tdh-01C, 2
Figure Note 2
SEC analysis of Threonine Dehydrogenase using a Superose 6 Increase 5x150 column 50 mM sodium phosphate; 0.3 M NaCl pH 7.2 at 100 µl/min
Figure Title 2
Size exclusion chromatography of ThrDH
Figure 3
tdh-01C, 3
Figure Note 3
Specific Activity of Threonine Dehydrogenase is ≥2 U/mg. In this reaction 1 µg of ThrDH converts L-Threonine into 2-amino-3-oxobutyrate with concomitant reduction of NAD+ which was measured at 340 nm.
Figure Title 3
Activity of Active ThrDH
