Cat.No.
Rev1-1532Y
Species
Yeast
Product Name
Recombinant yeast Rev1 protein
Product Overview
Recombinant full length Yeast Rev1 protein was expressed in E. coli.
Description
Yeast Rev1 is a member of the Y family DNA polymerases. It possesses the DNA template-dependent dCMP transferase. In yeast cells, Rev1 is required for
mutagenesis induced by several types of DNA damaging agents. In vitro, Rev1 is able to insert a C opposite several types of DNA lesions.
mutagenesis induced by several types of DNA damaging agents. In vitro, Rev1 is able to insert a C opposite several types of DNA lesions.
Source
E. coli
Molecular Mass
112 kDa
Usage
Reaction Buffer: 25 mM potassium phosphate (pH 7.0), 5 mM MgCl2, 5 mM DTT, 100 μg/ml BSA, 10% glycerol, 50-100 μM dNTPs.
Dilution Buffer: 25 mM Tris-HCl (pH 7.5), 2.5 mM β-mercaptoethanol, 50% glycerol.
Dilution Buffer: 25 mM Tris-HCl (pH 7.5), 2.5 mM β-mercaptoethanol, 50% glycerol.
