Cat.No.
NusA-391E
Species
E. coli
Product Name
Recombinant E.coli Transcription Termination/antitermination L Factor
Product Overview
Recombinant NusA was expressed in E.coli and purified by using conventional chromatography techniques, 54 kDa (495 aa).
Description
NusA is a key component in both Prevention and enhancement of transcriptional termination. It is important in both Rho-dependent and intrinsic termination, as well as in lambda and other phage antitermination systems. The gene was first identified by isolation of the nusAl mutation, which restricts bacteriophage l growth by preventing the antitermination activity of the l N protein. NusA is involved in transcriptional antitermination in the cell. It has been shown to specifically aid in read-through of the RNA polymerase genes rpoB and rpoC, as well as in successful synthesis of the ribosomal RNA genes.
Form
Liquid. In Phosphate Buffered Saline (pH 7.4)
Purity
> 95% by SDS - PAGE
Storage
Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Concentration
1 mg/ml (determined by Bradford assay)
Synonyms
ECK3158; JW3138; nusA; transcription termination/antitermination L factor; transcription pausing; L factor
Gene ID
Protein Refseq
