Cat.No.
Protease-20S
Species
S. griseus
Product Name
Native Streptomyces griseus Protease
Product Overview
It is collected from culture broth of S. griseus and purified by successive column procedures.
Description
A protease (also termed peptidase or proteinase) is any enzyme that performs proteolysis, that is, begins protein catabolism by hydrolysis of the peptide bonds that link amino acids together in the polypeptide chain forming the protein. Proteases have evolved multiple times, and different classes of protease can perform the same reaction by completely different catalytic mechanisms. Proteases can be found in animals, plants, bacteria, archea and viruses.
Source
Streptomyces griseus
Form
White to tan powder
Bio-activity
Approximately 4 units per mg solid.
Unit Definition
One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
Characteristic
Highly stable in pH range of 5.0 to 9.0, with peak activity at pH 8.8; Compatible with many DNA and RNA isolation buffers; Broad substrate specificity.
Applications
The enzyme has been used for the digestion and analysis of antithrombin-heparin complexes. It has also been used for the isolation of enzyme-resistant starch. This enzyme is more active at a higher pH range than the known alkaline protease, showing the proteolytic activity even in 0.2N NaOH solution. This enzyme is useful for proteolysis of insoluble protein and for structure investigation of protein.
Stability
Completely inactivated by heating above 80 °C for 15-20 minutes.
