Cat.No.
Mic-0080M
Product Name
Urea Broth
Product Details
Urea Broth is a liquid medium used for differentiating Enterobacteriaceae, especially Proteus, based on urease activity. This medium, buffered with Di- and Monopotassium Phosphates, contains Urea and Phenol Red as pH indicator. Urease activity alkalinizes the environment, thus producing a color shift from pale yellow to pink-red. Yeast Extract provides the nitrogenous sources essential for bacterial growth.
Product Ingredients
Yeast Extract (1.0 g/L), Urea (20.0 g/L), Dipotassium Phosphate (9.5 g/L), Phenol Red (0.01 g/L), Monopotassium Phosphate (9.1 g/L). Final pH: 6.8 ± 0.2 at 25 °C. Grams per liter may be adjusted or formula supplemented to obtain desired performance.
size
500g, 2kg, 10kg
Usage Method
Mix 39.6 grams of the medium in one liter of purified water until evenly dispersed. Heat with repeated stirring and boil for one minute to dissolve completely. Sterilize by filtration. DO NOT BOIL OR AUTOCLAVE.
Application
Food safety
Storage
Store the sealed bottle containing the dehydrated medium at 2 to 30°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original color.
Data Sheet
