Cat.No.
Mic-0078M
Product Name
Universal Pre-enrichment Broth
Product Details
Universal Pre-enrichment Broth is a non-selective medium used for the recovery of Salmonella and Listeria from food products. With traditional methods requiring different pre-enrichments for each microorganism, Universal Pre-enrichment Broth was formulated by Bailey and Cox for the recovery of sublethally injured strains of both Listeria and Salmonella from a single food product specimen. The medium is well buffered, containing Disodium and Monopotassium Phosphates, which allows the recovery of pH-sensitive bacteria. Enzymatic Digest of Casein and Protease Peptones supply necessary growth nutrients such as amino acids, peptides, vitamins, and electrolytes. Dextrose is an energy source to sustain bacterial growth. Sodium Chloride, Magnesium Sulfate and Ferric Ammonium Citrate provide essential electrolytes and maintain the osmotic equilibrium. Sodium Pyruvate stimulates the metabolism of stressed microorganisms.
Product Ingredients
Enzymatic Digest of Casein (5.0 g/L), Dextrose (0.5 g/L), Proteose Peptone (5.0 g/L), Sodium Chloride (5.0 g/L), Monopotassium Phosphate (15.0 g/L), Disodium Phosphate (7.0 g/L), Magnesium Sulfate (0.25 g/L), Ferric Ammonium Citrate (0.1 g/L), Sodium Pyruvate (0.2 g/L). Final pH: 6.3 ± 0.2 at 25 °C. Grams per liter may be adjusted or formula supplemented to obtain desired performance.
size
500g, 2kg, 10kg
Usage Method
Mix 38 grams of the medium in one liter of purified water until evenly dispersed. Heat with repeated stirring and boil for one minute to dissolve completely. Distribute and autoclave at 121°C for 15 minutes.
Application
Food safety
Storage
Store the sealed bottle containing the dehydrated medium at 2 to 30°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original color
Data Sheet
