Cat.No.
Mic-0073M
Product Name
Tryptose Phosphate Broth
Product Details
Tryptose Phosphate Broth is a liquid medium recommended for the cultivation of fastidious microorganisms. Enzymatic Digest of Casein, Soy Peptone, and Yeast Extract supply most of the nutrients for bacterial growth (amino acids, peptides, carbohydrates, vitamins, and minerals). Dextrose is the main carbohydrate source. Sodium Chloride provides essential electrolytes and maintains osmotic equilibrium. Disodium Phosphate acts as pH buffer. The addition of 0.1 - 0.2% Agar concentration can aid in the recovery of anaerobes.
Product Ingredients
Enzymatic Digest of Casein (10.0 g/L), Yeast Extract (5.0 g/L), Soy Peptone (5.0 g/L), Dextrose (2.0 g/L), Sodium Chloride (5.0 g/L), Disodium Phosphate (2.5 g/L). Final pH: 7.3 ± 0.2 at 25 °C. Grams per liter may be adjusted or formula supplemented to obtain desired performance.
size
500g, 2kg, 10kg
Usage Method
Mix 29.5 grams of the medium in one liter of purified water until evenly dispersed. Heat with repeated stirring and boil for one minute to dissolve completely. Distribute and autoclave at 121°C for 15 minutes.
Application
Food safety
Storage
Store the sealed bottle containing the dehydrated medium at 2 to 30°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original color.
Data Sheet
