Cat.No.
Mic-0071M
Product Name
Tetrathionate Broth Base
Product Details
Tetrathionate Broth Base, otherwise known as TT Broth, is used with an iodine-iodide solution for the cultivation of Salmonella spp. from clinical specimens, foods and other materials of sanitary importance. Enzymatic Digest of Casein and Animal Tissue supply nitrogenous substances and carbohydrates for bacterial growth. Upon addition of Iodine and Potassium Iodide, the Sodium Thiosulfate in this medium produces tetrathionate. Only microorganisms containing the enzyme tetrathionate reductase will proliferate in this medium. Bile Salts suppress the growth of coliforms and Gram-positive organisms. Calcium Carbonate neutralizes acid pH and absorbs toxic metabolites.
Product Ingredients
Enzymatic Digest of Casein (2.5g/L), Peptic Digest of Animal Tissue (2.5g/L), Calcium Carbonate (10.0g/L), Sodium Thiosulfate (30.0g/L), Bile Salts (1.0g/L). Final pH: 8.4 ± 0.2 at 25°C. Grams per liter may be adjusted or formula performance supplemented to obtain desired.
size
500g, 2kg, 10kg
Usage Method
Mix 46 grams of the medium in one liter of purified water until evenly dispersed. Heat with repeated stirring and boiling. Cool to 50.0 °C before adding 20ml of an iodine-iodide solution (6g iodine + 5g potassium iodide in 20ml of purified water). DO NOT REHEAT AND DO NOT AUTOCLAVE. Use immediately
Application
Food safety
Storage
Store the sealed bottle containing the dehydrated medium at 2 to 30°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original color.
Data Sheet
