Cat.No.
Mic-0068M
Product Name
TCBS Agar
Product Details
TCBS (Thiosulfate Citrate Bile Salts Sucrose) Agar medium is used for isolating and cultivating Vibrio cholerae and other enteropathogenic bacteria. TCBS is prepared according to the formula of Kobayashi et al. The pH of the medium is elevated to enhance the growth of V. cholerae. Sucrose is fermented by V. cholerae and its biotype Eltor producing yellowish-brown flat colonies. Fishbein et al. reported that V. parahaemolyticus produces light blueish colonies. Enteric and Gram-positive bacteria present in fecal specimens, including Proteus spp., are inhibited by the high concentrations of Sodium Thiosulfate and Citrate, the alkaline pH, and the presence of Bile Salts. TCBS Agar was also found useful in the isolation of V. fluvialis and V. vulnificus. Bromothymol Blue and Thymol Blue are pH indicators. Together with the fermentable sugar Sucrose, they allow to distinguish sucrose-fermenting (yellow colonies) and non-fermenting (green colonies) species.
Product Ingredients
Enzymatic Digest of Casein (10.0g/L), Yeast Extract (5.0g/L), Sodium Citrate (10.0g/L), Sodium Thiosulfate (10.0g/L), Oxgall (8.0g/L), Sucrose (20.0g/L), Sodium Chloride (10.0g/L), Ferric Ammonium Citrate (1.0g/L), Bromothymol Blue (0.04g/L), Agar (10.0g/L). Final pH: 8.6 ± 0.2 at 25°C. Grams per liter may be adjusted or formula supplemented to obtain desired performance.
size
500g, 2kg, 10kg
Usage Method
Mix 89 grams of the medium in one liter of purified water until evenly dispersed. Heat with repeated stirring to dissolve completely. DO NOT AUTOCLAVE.
Application
Food safety
Water treatment
Water treatment
Storage
Store the sealed bottle containing the dehydrated medium at 2 to 30°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original color.
Data Sheet
