Staphylococcus Agar #110

Mic-0067M

Product Name

Staphylococcus Agar #110

Product Details

Staphylococcus Agar is a selective medium for the isolation and differentiation of pathogenic Staphylococci. Enzymatic Digest of Casein and Yeast Extract provide amino acids, proteins, carbohydrates, vitamins, and electrolytes. D-Mannitol is the main carbohydrate source, while Lactose is the supplementary one. Dipotassium Phosphate acts as a pH buffer. Sodium Chloride provides electrolytes, maintains osmotic equilibrium, and makes the medium selective for Gram-positive bacteria. Gelatin is included to test liquefaction. Pathogenic Staphylococci grow well on this medium and form golden yellow colonies. Mannitol fermentation is detected by adding a few drops of Bromothymol Blue and looking for a yellow halo on the areas where colonies have been removed. Gelatin liquefaction can be observed as clear zones after flooding the plates with an aqueous solution of Ammonium Sulfate and incubating 10 min at 37 °C. Agar is the gelifying agent.

Product Ingredients

Casein Digest Peptone (10.0g/L), Yeast Extract (2.5g/L), D-Mannitol (10.0g/L), Dipotassium Phosphate (5.0g/L), Gelatin (30.0g/L), Lactose (2.0g/L), Sodium Chloride (75.0g/L), Agar (15.0g/L). Final pH: 7.0 ± 0.2 at 25°C. Grams per liter may be adjusted or formula supplemented to obtain desired performance.

size

500g, 2kg, 10kg

Usage Method

Mix 149 grams of the medium in a Liter of purified water until evenly dispersed. Heat with repeated stirring and boil for one minute to dissolve completely. Distribute and autoclave at 121.0°C for 15 minutes.

Application

Food safety
Cosmetic industry

Storage

Store the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.