Simmons Citrate Agar

Mic-0063M

Product Name

Simmons Citrate Agar

Product Details

Simmons Citrate Agar is used as a differential medium for the identification of enteric bacteria by using Citrate as the sole source of carbon and Ammonium as the sole source of nitrogen. Koser developed a fluid medium to determine citrate utilization, with Simmon further modifying the formula by adding 1.5% agar and Bromthymol Blue as an indicator. The medium can be used to distinguish between Escherichia coli and Enterobacter aerogenes and to assist in differentiating members of the Salmonella genus. Organisms able to metabolize the citrate (most Salmonella spp., some Enterobacteriaceae, most Klebsiella spp.) grow well on the surface of the medium and alkalinize the medium, changing it from green to blue. In turn, non-citrate fermenters (E. coli, Shigella spp.) are unable to shift the color.

Product Ingredients

Sodium Chloride (5.0 g/L), Magnesium Sulfate (0.2 g/L), Sodium Citrate (2.0 g/L), Bromothymol Blue (0.08 g/L), Ammonium Dihydrogen Phosphate (1.0 g/L), Agar (15.0 g/L), Potassium Phosphate Dibasic (1.0 g/L). Final pH: 6.9 ± 0.2 at 25 °C. Grams per liter may be adjusted or formula supplemented to obtain desired performance.

size

500g, 2kg, 10kg

Usage Method

Mix 24.3 grams of the medium in one liter of purified water until evenly dispersed. Heat with repeated stirring to dissolve completely. Distribute and autoclave at 121°C for 15 minutes. After autoclaving, place medium on a slant and allow for solidification.

Application

Food safety
Water treatment

Storage

Store the sealed bottle containing the dehydrated medium at 2 to 30°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original color.