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Selenite Broth

Cat.No.
Mic-0061M
Product Name
Selenite Broth
Product Details
A liquid enrichment used for isolating Salmonella typhi and other Salmonella spp. from a variety of sources such as feces, urine, and food. Enzymatic Digest of Casein and Animal Tissue supply nitrogenous substances and carbohydrates for bacterial growth. Sodium Phosphate stabilizes pH and reduces toxicity to Selenite. Sodium Selenite inhibits growth of Gram-positive microorganisms such as coliforms and fecal Streptococci. Lactose is the fermentable carbohydrate and maintains an optimal pH by neutralizing the alkalinization occurring upon Selenite reduction.
Product Ingredients
Enzymatic Digest of Casein (2.5g/L), Peptic Digest of Animal Tissue (2.5g/L), Sodium Phosphate (10.0g/L), Lactose (4.0g/L), Sodium Selenite (4.0g/L). Final pH: 7.0 ± 0.2 at 25°C. Grams per liter may be adjusted or formula supplemented to obtain desired performance.
size
500g, 2kg, 10kg
Usage Method
Mix 23.0 grams of the medium in one liter of purified water until evenly dispersed. Heat with repeated stirring to dissolve completely. DO NOT AUTOCLAVE.
Application
Food safety
Storage
Store the sealed bottle containing the dehydrated medium at 2 to 30°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original color.
Data Sheet
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