Cat.No.
Mic-0059M
Product Name
Salmonella Shigella Agar
Product Details
Salmonella Shigella Agar is used for isolating Salmonella spp. and some strains of Shigella spp. The medium is widely used in sanitary bacteriology to isolate these species from feces, urine, and fresh canned foods. The presence of Bile Salts No. 3 and Brilliant Green suppresses the growth of Gram-positive bacteria, most coliforms, and Proteus spp. The high Lactose content allows for the early detection of slow Lactose-fermenting microorganisms, while both Thiosulphate and Ferric Acid help identify H2S-producing bacteria. While Lactose-fermenting microorganisms produce red or pink colonies on a reddish background due to the color shift of the pH indicator Netural Red, Shigella forms transparent colonies. H2S-producing bacteria like Salmonella can be distinguished by the formation of colonies with black center due to the production of iron sulfide.
Product Ingredients
Beef Extract (5.0g/L), Meat Peptone (5.0g/L), Lactose (10.0g/L), Bile Salts No. 3 (8.5g/L), Sodium Citrate (8.5g/L), Sodium Thiosulfate (8.5g/L), Ferric Citrate (1.0g/L), Agar (13.5g/L), Brilliant Green (0.33mg/L), Neutral Red (0.025g/L). Final pH: 7.0 ± 0.2 at 25°C. Grams per liter may be adjusted or formula supplemented to obtain desired performance.
size
500g, 2kg, 10kg
Usage Method
Mix 60 grams of the medium in one liter of purified water until evenly dispersed. Heat with repeated stirring to dissolve completely. DO NOT AUTOCLAVE.
Application
Food safety
Storage
Store the sealed bottle containing the dehydrated medium at 2 to 30°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original pinkish-beige.
Data Sheet
