Cat.No.
CYC1-467Y
Species
Saccharomyces cerevisiae
Product Name
Recombinant Yeast Cyc1p
Product Overview
Recombinant Cytochrome c iso-1 from Yeast C107T mutant consists of the full length Cythochrome c protein (residues 1-108) bound to the heme group with Fe2+. MW = 12 KDa.
Description
Cytochrome c, or cyt c is a small heme protein found loosely associated with the inner membrane of the mitochondrion. It belongs to the cytochrome c family of proteins. Cytochrome c is a highly soluble protein, unlike other cytochromes, with a solubility of about 100g/L and is an essential component of the electron transport chain, where it carries one electron. It is capable of undergoing oxidation and reduction, but does not bind oxygen. It transfers electrons between Complexes III and IV.
Source
Yeast
Purity
> 95% by SDS-PAGE. The protein was observed as a single band migrating at a molecular weight of about 12 KDa. The purity is also checked through the ratio A415 nm/A280 nm.
Storage
-20º C. The protein is stable at 4 ºC for at least 2 weeks and at 25 ºC for at least several hours. After initial defrost, aliquot product into individual tubes and refreeze at -20º C. Avoid repeated freeze/defrost cycles.
Gene Name
Synonyms
CYC1; Cyc1p; Cytochrome c, isoform 1; UQCR4; Complex III subunit 4; Complex III subunit IV; Cytochrome b-c1 complex subunit 4; Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit; cytochrome c-1
Gene ID
Pathway
Pathways in cancer
Protein Refseq
Chromosome Location
8q24
Function
electron carrier activity; electron carrier activity; heme binding; iron ion binding; metal ion binding
Characteristic
Under the above described conditions, to avoid precipitation or protein dimerization, the product can be concentrated to a maximum of 1mM.
Supplied As
1.0 mg/ml in 20mM sodium phosphate pH 6.8, 1mM DTT. The concentration is calculated from the absorbance at 415nm (e415 = 122000M-1 cm-1).
Characteristics
Under the above described conditions, to avoid precipitation or protein dimerization, the product can be concentrated to a maximum of 1mM.
