Cat.No.
ICDH-81
Species
Saccharomyces cerevisiae
Product Name
Recombinant Saccharomyces Cerevisiae ICDH protein
Product Overview
Recombinant full length Saccharomyces Cerevisiae ICDH (NADP) was expressed in Yeast. The N-terminal amino acid Phenylalanine residue next to Met is substituted with Alanine for overexpression.
Description
Isocitrate Dehydrogenase is an enzyme of the oxidoreductase class that catalyzes the conversion of isocitrate and NAD+ to yield 2-ketoglutarate, carbon dioxide, and NADH. It occurs in cell mitochondria. The enzyme requires Mg2+, Mn2+; it is activated by ADP, citrate, and Ca2+, and inhibited by NADH, NADPH, and ATP. The reaction is the key rate-limiting step of the citric acid (tricarboxylic) cycle.
Source
Yeast
Form
One ml of solution (1mg/282ul)contains 0.075 mol/l KPO4, 50% Glycerol, pH 7.1.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Storage
Store at 4 centigrade if entire vial will be used within 2-4 weeks. Store, frozen at -20 centigrade for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Bio-activity
The specific activity was found to be 115 U/mg.
Unit Definition
One unit is defined as 1 µmol of NAD+ production per minute under the assay conditions (25 centigrade, pH 7.5).
